A ATUAÇÃO DA MEMBRANA AMNIÓTICA NA EXPRESSÃO DOS GENES CD206 E IL-12B NA FIBROSE HEPÁTICA BILIAR

Autores

DOI:

https://doi.org/10.18066/revistaunivap.v26i50.2340

Palavras-chave:

Expressão Gênica, fibrose hepática, macrófagos, membrana amniótica.

Resumo

A fibrose hepática é a consequência das doenças crônicas do fígado, e quando não tratada evolui para a cirrose, cujo único tratamento efetivo é o transplante do órgão. Estudos prévios demonstraram que a membrana amniótica humana (MA), reduziu a fibrose biliar inibindo sua progressão para a cirrose hepática. Acredita-se que um dos mecanismos desta ação seja a modulação dos macrófagos M1 e M2, células que atuam na progressão e regressão da fibrose, respectivamente. O objetivo deste estudo foi analisar o efeito da MA na expressão de genes relacionados aos macrófagos M1 e M2 na fibrose hepática por meio da técnica de PCR quantitativa em tempo real (RT-qPCR). Duas semanas após a indução da fibrose pela ligadura do ducto biliar (LDB), um fragmento de MA foi aplicado ao fígado. Quatro semanas após a aplicação da MA, amostras do fígado foram submetidas a extração do RNA, síntese de cDNA e RT-qPCR. Foi observado aumento significativo da expressão dos genes CD206, e IL-12b após a aplicação da MA em relação as amostras de fígado sem a presença da mesma, sugerindo que a MA pode estimular a expressão do gene CD206, que está associado ao macrófago M2, e reparação da fibrose, mas também pode ter o potencial de estimular a expressão do gene IL-12b, associada ao macrófago M1. Estes resultados sugerem a presença de ambos os subtipos de macrófagos durante a regressão da fibrose biliar, a qual foi evidenciada previamente pelo nosso grupo de pesquisa, utilizando os mesmos grupos experimentais. Assim, estudos futuros com outros genes contribuirão para o melhor entendimento da ação antifibrótica da MA por meio do aumento de M2 no tecido hepático.

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Biografia do Autor

Roberta Jenniffer Maciel Teixeira, Universidade do Vale do Paraíba - Unviap

Graduada em Odontologia pela Universidade do Vale do Paraíba - Univap.

Raissa Monteiro da Silva, Universidade do Vale do Paraíba - Unviap

Graduanda de Biomedicina pela Universidade do Vale do Paraíba - Univap.

Renata de Azevedo Carnevari, Universidade do Vale do Paraíba - Unviap

Professora Doutora da Universidade do Vale do Paraíba e coordenadora do Laboratório de Biologia Molecular do Câncer - IP&D, Universidade do Vale do Paraíba.

Luciana Barros Sant'Anna, Universidade do Vale do Paraíba - Unviap

Professora Doutora da Universidade do Vale do Paraíba e coordenadora do Laboratório de Histologia e Terapia Regenerativa - IP&D, Universidade do Vale do Paraíba.

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Publicado

2020-07-01

Como Citar

Maciel Teixeira, R. J., Alves, A. P. da S., Silva, R. M. da, Carnevari, R. de A., & Sant’Anna, L. B. (2020). A ATUAÇÃO DA MEMBRANA AMNIÓTICA NA EXPRESSÃO DOS GENES CD206 E IL-12B NA FIBROSE HEPÁTICA BILIAR. Revista Univap, 26(50), 47–63. https://doi.org/10.18066/revistaunivap.v26i50.2340

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