ANALYSIS OF COLLAGEN PRODUCTION IN HYPERTROPHIC CARDIAC TISSUE OF RATS TREATED WITH L-NAME AND L-ARGININE BY IMAGE PROCESSING
DOI:
https://doi.org/10.18066/revistaunivap.v25i48.2283
Abstract
We analyzed the production of collagen in the heart of hypertensive rats induced by L-name. Methods: Cardiac imaging of 5 groups: NT, hypertension induced by L-name, L-name and 10 mg L-arginine, L-name and 30 mg L-arginine and L-name and 100 mg L-arginine. Quantification of COL by histology with picrosirius CFC_Image v. 2.0. Calibration of the software by selecting values of hue, saturation and HSI intensity in the reference image, use of the software in the images used. Results: The images did not present statistical differences in any of the HSI sets, indicating that the calibration of the software is valid for the study. Doses greater than 30 mg of L-arginine showed a greater reduction in the collagen areas, indicating a greater protection of the cardiac structures in hypertension. Conclusion: CFC_Image v. 2.0. The accuracy demonstrated in the quantification of COL, L-arginine supplementation promoted protective effects in murine models of hypertension.
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References
BERNÁTOVÁ, I. et al. Chronic low-dose L-NAME treatment increases nitric oxide production and vasorelaxation in normotensive rats. Physiol. Res., v. 56, n. 2, p. S17, 2007.
BOCCHI, E. A. et al. III Brazilian Guideline for Chronic Heart Failure. Arq Bras Cardiol., v. 93, n. 1, p. 3-70, 2009.
BRASIL. Ministério da Saúde. Hipertensão arterial sistêmica para o Sistema Único de Saúde. Brasília: Ministério da Saúde, 2006. (Cadernos de Atenção Básica, n. 15) (Série A. Normas e Manuais Técnicos).
CAETANO, J. A. et al. Description of risk factors for cardiovascular disorders in a group of elderly people. Text. Context. Enferm., v. 17, n. 2, p. 327-35, 2008.
CALAIS, G. S. P.; GARCIA, G. C.; CALDEIRA, T. R. Hipertensão arterial. Saúde e Economia, ano. 2, n. 4, 2010. Available at: http://portal.anvisa.gov.br/documents/33884/412285/Boletim+Sa%C3%BAde+e+Economia+n%C2%BA+4. Accessed on: 12 Feb. 2019.
CARMO, E. C. et al. The association of anabolic steroids with physical-aerobic training leads to cardiac morphological changes and loss of ventricular function in rats. Ver. Bras. Med. Esporte, v. 17, n. 2, p. 137-141, Apr. 2011.
JUNQUEIRA, L.C.; BIGNOLAS, G.; BRENTANI, R. R. Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Histochem. J., v. 11, n. 4, p. 447-455, 1979.
LIEBSON, P. R. et al. Echocardiographic correlates of left ventricular structure among 844 mildly hypertensive men and women in the Treatment of Mild Hypertension Study (TOMHS). Circulation, v. 87, n. 2, p. 476-486, 1993.
MATSUOKA, H. et al. Chronic L-arginine administration attenuates cardiac hypertrophy in spontaneously hypertensive rats. Hypertension, v. 27, n. 1, p. 14-18, 1996.
MELO-JÚNIOR, M. R. et al. Digital image analysis in pathology-the interface with Biomedical Engineering. Rev. Bras. Eng. Bioméd., v. 22, n. 3, p. 239-242, 2006.
MORENO, H JR. et al. Chronic nitric oxide inhibition as a model of hypertensive heart muscle disease. Basic Res Cardiol., v. 91, n. 3, p. 248-55, 1996.
PEREIRA, L. M. M.; VIANNA, G. M. M.; MANDARIM-DE-LACERDA, C. A. Morphology and myocardial stereology in hypertensive rats. Correlation with time of inhibition of nitric oxide synthesis. Arq. Bras. Cardiol, v. 70, n. 6, p. 397-402, 1998.
RAMOS, L. Estudo hemodinâmico e morfológico do miocárdio de ratos hipertensos. Correlação com tratamento preventivo com L-arginina. São José dos Campos, SP, 2005. Dissertação (Mestrado em Ciências Biológicas) - Universidade do Vale do Paraíba, Instituto de Pesquisa e Desenvolvimento, São José dos Campos, 2005.
RIBEIRO, M.O. et al. Chronic inhibition of nitric oxide synthesis. A new model of arterial hypertension. Hypertension, v. 20, n. 3, p. 298-303, 1992.
TSAI, S. H. et al. Enhanced endothelin-1/Rho-kinase signalling and coronary microvascular dysfunction in hypertensive myocardial hypertrophy. Cardiovasc Res., v. 113, n. 11, p. 1329-1337, 2017.
ZATZ, R.; DE NUCCI, G. Effects of acute nitric oxide inhibition on rat glomerular microcirculation. Am. J. Physiol., v. 261, n. 2, p. 360-363, 1991.
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2019-12-06
How to Cite
Marson, V. O. C., Bissaco, M. A. S., Ramos, L., Rodrigues, S. C. M., Da Silva, R. T., Mafra, F. F. P., Macedo, M. M., Martins, P. S. L. L., & Martins, R. A. B. L. (2019). ANALYSIS OF COLLAGEN PRODUCTION IN HYPERTROPHIC CARDIAC TISSUE OF RATS TREATED WITH L-NAME AND L-ARGININE BY IMAGE PROCESSING. Revista Univap, 25(48), 24–31. https://doi.org/10.18066/revistaunivap.v25i48.2283
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Artigos da edição especial "Vivendo globalmente e agindo localmente - A saúde como tema complexo"
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This work is licensed under a Creative Commons Attribution 4.0 International.
This license allows others to distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation.
http://creativecommons.org/licenses/by/4.0/legalcode
DOI:
https://doi.org/10.18066/revistaunivap.v25i48.2283Abstract
We analyzed the production of collagen in the heart of hypertensive rats induced by L-name. Methods: Cardiac imaging of 5 groups: NT, hypertension induced by L-name, L-name and 10 mg L-arginine, L-name and 30 mg L-arginine and L-name and 100 mg L-arginine. Quantification of COL by histology with picrosirius CFC_Image v. 2.0. Calibration of the software by selecting values of hue, saturation and HSI intensity in the reference image, use of the software in the images used. Results: The images did not present statistical differences in any of the HSI sets, indicating that the calibration of the software is valid for the study. Doses greater than 30 mg of L-arginine showed a greater reduction in the collagen areas, indicating a greater protection of the cardiac structures in hypertension. Conclusion: CFC_Image v. 2.0. The accuracy demonstrated in the quantification of COL, L-arginine supplementation promoted protective effects in murine models of hypertension.
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References
BERNÁTOVÁ, I. et al. Chronic low-dose L-NAME treatment increases nitric oxide production and vasorelaxation in normotensive rats. Physiol. Res., v. 56, n. 2, p. S17, 2007.
BOCCHI, E. A. et al. III Brazilian Guideline for Chronic Heart Failure. Arq Bras Cardiol., v. 93, n. 1, p. 3-70, 2009.
BRASIL. Ministério da Saúde. Hipertensão arterial sistêmica para o Sistema Único de Saúde. Brasília: Ministério da Saúde, 2006. (Cadernos de Atenção Básica, n. 15) (Série A. Normas e Manuais Técnicos).
CAETANO, J. A. et al. Description of risk factors for cardiovascular disorders in a group of elderly people. Text. Context. Enferm., v. 17, n. 2, p. 327-35, 2008.
CALAIS, G. S. P.; GARCIA, G. C.; CALDEIRA, T. R. Hipertensão arterial. Saúde e Economia, ano. 2, n. 4, 2010. Available at: http://portal.anvisa.gov.br/documents/33884/412285/Boletim+Sa%C3%BAde+e+Economia+n%C2%BA+4. Accessed on: 12 Feb. 2019.
CARMO, E. C. et al. The association of anabolic steroids with physical-aerobic training leads to cardiac morphological changes and loss of ventricular function in rats. Ver. Bras. Med. Esporte, v. 17, n. 2, p. 137-141, Apr. 2011.
JUNQUEIRA, L.C.; BIGNOLAS, G.; BRENTANI, R. R. Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Histochem. J., v. 11, n. 4, p. 447-455, 1979.
LIEBSON, P. R. et al. Echocardiographic correlates of left ventricular structure among 844 mildly hypertensive men and women in the Treatment of Mild Hypertension Study (TOMHS). Circulation, v. 87, n. 2, p. 476-486, 1993.
MATSUOKA, H. et al. Chronic L-arginine administration attenuates cardiac hypertrophy in spontaneously hypertensive rats. Hypertension, v. 27, n. 1, p. 14-18, 1996.
MELO-JÚNIOR, M. R. et al. Digital image analysis in pathology-the interface with Biomedical Engineering. Rev. Bras. Eng. Bioméd., v. 22, n. 3, p. 239-242, 2006.
MORENO, H JR. et al. Chronic nitric oxide inhibition as a model of hypertensive heart muscle disease. Basic Res Cardiol., v. 91, n. 3, p. 248-55, 1996.
PEREIRA, L. M. M.; VIANNA, G. M. M.; MANDARIM-DE-LACERDA, C. A. Morphology and myocardial stereology in hypertensive rats. Correlation with time of inhibition of nitric oxide synthesis. Arq. Bras. Cardiol, v. 70, n. 6, p. 397-402, 1998.
RAMOS, L. Estudo hemodinâmico e morfológico do miocárdio de ratos hipertensos. Correlação com tratamento preventivo com L-arginina. São José dos Campos, SP, 2005. Dissertação (Mestrado em Ciências Biológicas) - Universidade do Vale do Paraíba, Instituto de Pesquisa e Desenvolvimento, São José dos Campos, 2005.
RIBEIRO, M.O. et al. Chronic inhibition of nitric oxide synthesis. A new model of arterial hypertension. Hypertension, v. 20, n. 3, p. 298-303, 1992.
TSAI, S. H. et al. Enhanced endothelin-1/Rho-kinase signalling and coronary microvascular dysfunction in hypertensive myocardial hypertrophy. Cardiovasc Res., v. 113, n. 11, p. 1329-1337, 2017.
ZATZ, R.; DE NUCCI, G. Effects of acute nitric oxide inhibition on rat glomerular microcirculation. Am. J. Physiol., v. 261, n. 2, p. 360-363, 1991.
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License
This work is licensed under a Creative Commons Attribution 4.0 International.
This license allows others to distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation.
http://creativecommons.org/licenses/by/4.0/legalcode
